Beauveria bassiana

Mitosporitic fungus:

Previously classified as: Deuteromycetes: Moniliales

Approved name:

Beauveria bassiana (Balsamo) Vuillemin.

Other names:

Previously known as Botrytis bassiana Balsamo. (White muscardine).


An isolate of Beauveria bassiana was obtained from a mycosed larva of the European corn borer (Ostrinia nubilalis (Hubner)) found in Beauce, France by INRA. A production process was developed by INRA and is now owned by Natural Plant Protection (NPP). Similarly, the Troy isolate was obtained from a boll weevil (Anthonomus grandis (Boheman)) at the USDA-ARS Crop Insect Research Center, Lower Rio Grande Valley, Texas. The three isolates that have been commonly commercialised are Bb 147 - NPP; ATCC 74040 (= ARSEF 3097 = FCI 7744) - Troy; and GHA - Mycotech.


Beauveria bassiana is cultured by semi-solid as well as by solid state fermentation on clay granules.


Strain Bb 147 is most commonly being used against borers, European Corn borer (Ostrinlo nubilalis)and Asiatic corn borer (O furnacalis Guenee); different strain (GHA) is also being used against whitefly, thrips, aphids and mealybugs; and ATCC 74040 is effective against a range of soft-bodied coleopteran, homopteran and heteropteran pests.


Corn, Coffee, Cotton and Sugarcane



The entomopathogen invades the insect body. Fungal conidia become attached to the insect cuticle and, after germination, the hyphae penetrate the cuticle and proliferate in the insect's body. High humidity or free water is essential for conidial germination and infection establishes between 24 and 48 hours. The infected insect may live for three to five days after hyphal penetration and, after death, the conidiophores bearing conidia are produced on cadaver. The fungus is insect specific.



Clay microgranular formulation colonised by sporulating mycelia.




Used as foliar sprays. Application rates depend upon the crop and the pests to be controlled. The normal application rate on commodity crops is 750 to 1000 ml of product per hectare, for ornamentals under cover or outdoors 24 to 80 ml per 10 litres and on turf and lawns 32 to 96 ml per 100 square metres.



Formulations should contain conidia of Beauveria bassiana at a concentration of 2.3 x 107 spores per ml or at least 5 x 108 spores per gram. Viability of the spores is determined by culture on nutrient agar and counting the colonies formed. Efficacy is checked by bioassay with an appropriate insect. Detailed identification of the specific strain in any formulation requires DNA and isoenzyme matching with the registered strain held in a type culture collection.

Storage conditions and shelf-life:

Store in a cool, dry place. Do not freeze and do not allow the product to undergo thermal shock. May be kept for up to one year if stored below 20 °C.


The products may be used alone or tank mixed with other products such as sticking agents, insecticidal soaps, emulsifiable oils, insecticides or used with beneficial insects. Do not use with fungicides and wait 48 hours after application before applying fungicides.



Oral LD50: (5 days) quail >2,000 mg/kg daily (by gavage).


Naturalis-L does not affect fish embryos, larvae or adults. LC50: (31 days) rainbow trout 7,300 mg/litre. EC50: (14 days) Daphnia pulex 4,100 mg/litre. Bees: 30-day dietary and contact studies indicate that Naturalis-L has no significant effect; LC50: (23 days, ingestion) 9,285 mg/kg.

Other beneficial species:

Field application did not result in adverse effect on beneficial insects. 


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